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            <title xml:lang="en">Comparison of Phytochemical Composition and Biological Activities of Rubus ulmifolius Extracts Originating from Four Regions of Tunisia</title>
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                <term xml:lang="en">Rubus ulmifolius</term>
                <term xml:lang="en"> Antibacterial activity</term>
                <term xml:lang="en"> Antioxidant activity</term>
                <term xml:lang="en"> Chemical composition</term>
                <term xml:lang="en"> Secondary metabolites</term>
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              <p>In the current study, the phenolic composition, antioxidant and antimicrobial activities of extracts from Rubus ulmifolius Schott leaves harvested in four localities (Sejnen, Tabarka, Faija and Ain drahem) in Tunisia were investigated for the first time. Great differences were found for the chemical composition, total phenol contents and biological activities among the evaluated extracts. HPLC analysis of methanolic extracts showed that the dominant compounds were kaempferol 3-O-rutinoside and naringenine. In addition, significant correlations were observed between antioxidant activities and phenolic contents. In fact, leaves collected from Sejnen presented higher total phenol content (53.32 mg GAE/g DW) and antioxidant activities (IC50 = 39.40 mg/l) than the others samples. All extracts showed significant antimicrobial activity against six used bacteria with the inhibition zones diameters and minimal inhibitory concentration values were in the range of 8 - 16 mm and 6.25 - 25 mg/ml, respectively. The highest antimicrobial activities were recorded in Sejnen extract against Gram-positive bacteria.</p>
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