<?xml version="1.0" encoding="utf-8"?>
<TEI xmlns="http://www.tei-c.org/ns/1.0" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:hal="http://hal.archives-ouvertes.fr/" xmlns:gml="http://www.opengis.net/gml/3.3/" xmlns:gmlce="http://www.opengis.net/gml/3.3/ce" version="1.1" xsi:schemaLocation="http://www.tei-c.org/ns/1.0 http://api.archives-ouvertes.fr/documents/aofr-sword.xsd">
  <teiHeader>
    <fileDesc>
      <titleStmt>
        <title>HAL TEI export of hal-03591838</title>
      </titleStmt>
      <publicationStmt>
        <distributor>CCSD</distributor>
        <availability status="restricted">
          <licence target="https://creativecommons.org/publicdomain/zero/1.0/">CC0 1.0 - Universal</licence>
        </availability>
        <date when="2026-05-24T20:31:45+02:00"/>
      </publicationStmt>
      <sourceDesc>
        <p part="N">HAL API Platform</p>
      </sourceDesc>
    </fileDesc>
  </teiHeader>
  <text>
    <body>
      <listBibl>
        <biblFull>
          <titleStmt>
            <title xml:lang="en">Development of support based on chitosan and cellulose nanocrystals for the immobilization of anti-Shiga toxin 2B antibody</title>
            <author role="aut">
              <persName>
                <forename type="first">Shiv</forename>
                <surname>Shankar</surname>
              </persName>
              <idno type="halauthorid">1642565-0</idno>
              <affiliation ref="#struct-54664"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Amina</forename>
                <surname>Baraketi</surname>
              </persName>
              <idno type="halauthorid">1642567-0</idno>
              <affiliation ref="#struct-54664"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Sabato</forename>
                <surname>d'Auria</surname>
              </persName>
              <idno type="halauthorid">1586922-0</idno>
              <affiliation ref="#struct-1088445"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Carole</forename>
                <surname>Fraschini</surname>
              </persName>
              <idno type="halauthorid">707785-0</idno>
              <affiliation ref="#struct-54664"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Stephane</forename>
                <surname>Salmieri</surname>
              </persName>
              <idno type="halauthorid">707778-0</idno>
              <affiliation ref="#struct-54664"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Majid</forename>
                <surname>Jamshidian</surname>
              </persName>
              <idno type="idhal" notation="numeric">776888</idno>
              <idno type="halauthorid" notation="string">996824-776888</idno>
              <idno type="IDREF">https://www.idref.fr/168742047</idno>
              <affiliation ref="#struct-54664"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Marie Christine</forename>
                <surname>Etty</surname>
              </persName>
              <idno type="halauthorid">2440428-0</idno>
              <affiliation ref="#struct-54664"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Monique</forename>
                <surname>Lacroix</surname>
              </persName>
              <idno type="halauthorid">374804-0</idno>
              <affiliation ref="#struct-54664"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Sabato</forename>
                <surname>D’auria</surname>
              </persName>
              <idno type="halauthorid">1903713-0</idno>
              <affiliation ref="#struct-54664"/>
            </author>
            <editor role="depositor">
              <persName>
                <forename>Michel</forename>
                <surname>Courcelles</surname>
              </persName>
              <email type="md5">235ee71abfb5f3fb19e1b204192e01ee</email>
              <email type="domain">iaf.inrs.ca</email>
            </editor>
          </titleStmt>
          <editionStmt>
            <edition n="v1" type="current">
              <date type="whenSubmitted">2022-02-28 19:48:20</date>
              <date type="whenModified">2024-12-19 11:12:04</date>
              <date type="whenReleased">2022-02-28 19:48:20</date>
              <date type="whenProduced">2020-03</date>
            </edition>
            <respStmt>
              <resp>contributor</resp>
              <name key="157387">
                <persName>
                  <forename>Michel</forename>
                  <surname>Courcelles</surname>
                </persName>
                <email type="md5">235ee71abfb5f3fb19e1b204192e01ee</email>
                <email type="domain">iaf.inrs.ca</email>
              </name>
            </respStmt>
          </editionStmt>
          <publicationStmt>
            <distributor>CCSD</distributor>
            <idno type="halId">hal-03591838</idno>
            <idno type="halUri">https://hal.science/hal-03591838</idno>
            <idno type="halBibtex">shankar:hal-03591838</idno>
            <idno type="halRefHtml">&lt;i&gt;Carbohydrate Polymers&lt;/i&gt;, 2020, 232, pp.115785. &lt;a target="_blank" href="https://dx.doi.org/10.1016/j.carbpol.2019.115785"&gt;&amp;#x27E8;10.1016/j.carbpol.2019.115785&amp;#x27E9;&lt;/a&gt;</idno>
            <idno type="halRef">Carbohydrate Polymers, 2020, 232, pp.115785. &amp;#x27E8;10.1016/j.carbpol.2019.115785&amp;#x27E9;</idno>
            <availability status="restricted"/>
          </publicationStmt>
          <seriesStmt>
            <idno type="stamp" n="RIIP">Pasteur Network</idno>
            <idno type="stamp" n="INRS-IAF" corresp="RIIP">Institut Armand Frappier</idno>
          </seriesStmt>
          <notesStmt>
            <note type="audience" n="2">International</note>
            <note type="popular" n="0">No</note>
            <note type="peer" n="1">Yes</note>
          </notesStmt>
          <sourceDesc>
            <biblStruct>
              <analytic>
                <title xml:lang="en">Development of support based on chitosan and cellulose nanocrystals for the immobilization of anti-Shiga toxin 2B antibody</title>
                <author role="aut">
                  <persName>
                    <forename type="first">Shiv</forename>
                    <surname>Shankar</surname>
                  </persName>
                  <idno type="halauthorid">1642565-0</idno>
                  <affiliation ref="#struct-54664"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Amina</forename>
                    <surname>Baraketi</surname>
                  </persName>
                  <idno type="halauthorid">1642567-0</idno>
                  <affiliation ref="#struct-54664"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Sabato</forename>
                    <surname>d'Auria</surname>
                  </persName>
                  <idno type="halauthorid">1586922-0</idno>
                  <affiliation ref="#struct-1088445"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Carole</forename>
                    <surname>Fraschini</surname>
                  </persName>
                  <idno type="halauthorid">707785-0</idno>
                  <affiliation ref="#struct-54664"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Stephane</forename>
                    <surname>Salmieri</surname>
                  </persName>
                  <idno type="halauthorid">707778-0</idno>
                  <affiliation ref="#struct-54664"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Majid</forename>
                    <surname>Jamshidian</surname>
                  </persName>
                  <idno type="idhal" notation="numeric">776888</idno>
                  <idno type="halauthorid" notation="string">996824-776888</idno>
                  <idno type="IDREF">https://www.idref.fr/168742047</idno>
                  <affiliation ref="#struct-54664"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Marie Christine</forename>
                    <surname>Etty</surname>
                  </persName>
                  <idno type="halauthorid">2440428-0</idno>
                  <affiliation ref="#struct-54664"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Monique</forename>
                    <surname>Lacroix</surname>
                  </persName>
                  <idno type="halauthorid">374804-0</idno>
                  <affiliation ref="#struct-54664"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Sabato</forename>
                    <surname>D’auria</surname>
                  </persName>
                  <idno type="halauthorid">1903713-0</idno>
                  <affiliation ref="#struct-54664"/>
                </author>
              </analytic>
              <monogr>
                <idno type="halJournalId" status="VALID">11513</idno>
                <idno type="issn">0144-8617</idno>
                <idno type="eissn">1879-1344</idno>
                <title level="j">Carbohydrate Polymers</title>
                <imprint>
                  <publisher>Elsevier</publisher>
                  <biblScope unit="volume">232</biblScope>
                  <biblScope unit="pp">115785</biblScope>
                  <date type="datePub">2020-03</date>
                </imprint>
              </monogr>
              <idno type="doi">10.1016/j.carbpol.2019.115785</idno>
              <idno type="pubmed">31952593</idno>
            </biblStruct>
          </sourceDesc>
          <profileDesc>
            <langUsage>
              <language ident="en">English</language>
            </langUsage>
            <textClass>
              <keywords scheme="author">
                <term xml:lang="en">Fractional factorial design</term>
                <term xml:lang="en">Chitosan</term>
                <term xml:lang="en">Cellulose nanocrystals</term>
                <term xml:lang="en">Box-Behnken design</term>
                <term xml:lang="en">Antibody immobilization</term>
              </keywords>
              <classCode scheme="mesh">Antibodies, Monoclonal</classCode>
              <classCode scheme="mesh">Antigen-Antibody Reactions</classCode>
              <classCode scheme="mesh">Cellulose</classCode>
              <classCode scheme="mesh">Chitosan</classCode>
              <classCode scheme="mesh">Nanostructures</classCode>
              <classCode scheme="mesh">Shiga Toxin</classCode>
              <classCode scheme="halDomain" n="sdv">Life Sciences [q-bio]</classCode>
              <classCode scheme="halTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halOldTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halTreeTypology" n="ART">Journal articles</classCode>
            </textClass>
            <abstract xml:lang="en">
              <p>This work describes the development of membrane based on chitosan (CHI), cellulose nanocrystals (CNCs), and glycerol (GLY), and optimization of the formulation for immobilization of monoclonal anti-Shiga toxin 2B antibody (mAnti-stx2B-Ab) for E. coli O157:H7 detection. The effect of CHI deacetylation degree &amp; viscosity, CNCs and GLY concentrations on Anti-stx2B-Ab immobilization efficiency was evaluated. Fractional factorial and Box-Behnken designs were applied to screen the effects of compounds interactions and optimize their concentrations for detection of Anti-stx2B-Ab. The results demonstrated that the use of 0.6 % (w/v) CNCs improved significantly the Anti-stx2B-Ab immobilization and the level of signal detection. The detection limit of Escherichia coli O157:H7 by developed optimized membrane is 1 log CFU/mL. The time needed for detection of E. coli O157:H7 was only 4 h of enrichment compared to 24 h with conventional methods. The developed immobilization support has potential for future pathogen detection in food and biomedical samples.</p>
            </abstract>
          </profileDesc>
        </biblFull>
      </listBibl>
    </body>
    <back>
      <listOrg type="structures">
        <org type="regrouplaboratory" xml:id="struct-54664" status="VALID">
          <orgName>Armand-Frappier Santé Biotechnologie Research Centre</orgName>
          <orgName type="acronym">INRS-AFSB</orgName>
          <date type="start">1938-01-01</date>
          <desc>
            <address>
              <addrLine>531 boul. des Prairies Laval (Québec) H7V 1B7</addrLine>
              <country key="CA"/>
            </address>
            <ref type="url">https://inrs.ca/en/inrs/research-centres/armand-frappier-sante-biotechnologie-research-centre/</ref>
          </desc>
          <listRelation>
            <relation active="#struct-92448" type="direct"/>
            <relation active="#struct-301247" type="direct"/>
          </listRelation>
        </org>
        <org type="institution" xml:id="struct-1088445" status="INCOMING">
          <orgName>Institute of Food Sciences National Research Council</orgName>
          <orgName type="acronym">ISA-CNR</orgName>
          <desc>
            <address>
              <country key="IT"/>
            </address>
          </desc>
        </org>
        <org type="institution" xml:id="struct-92448" status="VALID">
          <idno type="ROR">https://ror.org/04td37d32</idno>
          <orgName>Institut National de la Recherche Scientifique [Québec]</orgName>
          <orgName type="acronym">INRS</orgName>
          <desc>
            <address>
              <addrLine>490 rue de la Couronne, Québec, QC G1K 9A9</addrLine>
              <country key="CA"/>
            </address>
            <ref type="url">http://www.inrs.ca/</ref>
          </desc>
        </org>
        <org type="regroupinstitution" xml:id="struct-301247" status="VALID">
          <orgName>Pasteur Network (Réseau International des Instituts Pasteur)</orgName>
          <desc>
            <address>
              <addrLine>25, rue du Dr Roux 75724 Paris Cedex 15</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">https://pasteur-network.org/</ref>
          </desc>
        </org>
      </listOrg>
    </back>
  </text>
</TEI>