Single-Cell Electrochemical Aptasensor Array - RIIP - Réseau International des Instituts Pasteur Accéder directement au contenu
Article Dans Une Revue ACS Sensors Année : 2023

Single-Cell Electrochemical Aptasensor Array

Résumé

Despite several demonstrations of electrochemical devices with limits of detection (LOD) of 1 cell/mL, the implementation of single-cell bioelectrochemical sensor arrays has remained elusive due to the challenges of scaling up. In this study, we show that the recently introduced nanopillar array technology combined with redox-labelled aptamers targeting epithelial cell adhesion molecule (EpCAM) is perfectly suited for such implementation. Combining nanopillar arrays with microwells determined for single cell trapping directly on the sensor surface, single target cells are successfully detected and analyzed. This first implementation of a single-cell electrochemical aptasensor array, based on Brownian-fluctuating redox species, opens new opportunities for large-scale implementation and statistical analysis of early cancer diagnosis and cancer therapy in clinical settings.
Fichier principal
Vignette du fichier
2023.03.16.532912v2.full.pdf (1.92 Mo) Télécharger le fichier
Origine Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-04289140 , version 1 (16-11-2023)

Identifiants

Citer

Shuo Li, Yannick Coffinier, Chann Lagadec, Fabrizio Cleri, Katsuhiko Nishiguchi, et al.. Single-Cell Electrochemical Aptasensor Array. ACS Sensors, 2023, 8 (8), pp.2921-2926. ⟨10.1021/acssensors.3c00570⟩. ⟨hal-04289140⟩
56 Consultations
13 Téléchargements

Altmetric

Partager

Gmail Mastodon Facebook X LinkedIn More