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            <title xml:lang="en">SARS coronavirus 8b reduces viral replication by down-regulating E via an ubiquitin-independent proteasome pathway.</title>
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                <forename type="first">Choong-Tat</forename>
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            <idno type="halRefHtml">&lt;i&gt;Microbes and Infection&lt;/i&gt;, 2011, 13 (2), pp.179-88. &lt;a target="_blank" href="https://dx.doi.org/10.1016/j.micinf.2010.10.017"&gt;&amp;#x27E8;10.1016/j.micinf.2010.10.017&amp;#x27E9;&lt;/a&gt;</idno>
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                    <forename type="first">Yee-Joo</forename>
                    <surname>Tan</surname>
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                  <biblScope unit="volume">13</biblScope>
                  <biblScope unit="issue">2</biblScope>
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                  <date type="datePub">2011-02</date>
                  <date type="dateEpub">2010-10-28</date>
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              <classCode scheme="mesh">Animals</classCode>
              <classCode scheme="mesh">Cercopithecus aethiops</classCode>
              <classCode scheme="mesh">SARS Virus</classCode>
              <classCode scheme="mesh">Ubiquitin</classCode>
              <classCode scheme="mesh">Ubiquitination</classCode>
              <classCode scheme="mesh">Vero Cells</classCode>
              <classCode scheme="mesh">Viral Envelope Proteins</classCode>
              <classCode scheme="mesh">Viral Proteins</classCode>
              <classCode scheme="mesh">Virus Replication</classCode>
              <classCode scheme="mesh">Coronavirus Infections</classCode>
              <classCode scheme="mesh">Down-Regulation</classCode>
              <classCode scheme="mesh">Gene Expression Regulation, Viral</classCode>
              <classCode scheme="mesh">Gene Knockout Techniques</classCode>
              <classCode scheme="mesh">Green Fluorescent Proteins</classCode>
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              <p>The severe acute respiratory syndrome coronavirus (SARS-CoV) 8b protein, which is not expressed by other known coronaviruses, can down-regulate the envelope (E) protein via a proteasome-dependent pathway. Here, we showed that the down-regulation of E is not dependent on the lysine residues on 8b and the reduction of polyubiquitination of E mutants is not correlated with their down-regulation by 8b, suggesting an ubiquitin-independent proteasome pathway is involved. A time-course study revealed that 8b was expressed at late-stages of SARS-CoV infection. By using Vero E6 cells stably expressing green fluorescence protein-tagged 8b, ectopic expression of 8b was shown to significantly reduce the production of progeny virus and down-regulate E expression. Taken together, these results suggest that 8b negatively modulates virus replication by down-regulating E via an ubiquitin-independent proteasome pathway.</p>
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