%0 Journal Article %T Molecular detection of a novel human influenza (H1N1) of pandemic potential by conventional and real-time quantitative RT-PCR assays. %+ Department of Microbiology [HKU] %+ Department of Microbiology, Queen Mary Hospital %+ Centre de recherche Université de Hong-Kong-Pasteur %A Poon, Leo L M %A Chan, K. H. %A Smith, G. J. %A Leung, C. S. W. %A Guan, Y. %A Yuen, K. Y. %A Peiris, J. S. M. %< avec comité de lecture %@ 0009-9147 %J Clinical Chemistry %I American Association for Clinical Chemistry %V 55 %N 8 %P 1555-8 %8 2009-08 %D 2009 %R 10.1373/clinchem.2009.130229 %M 19439731 %Z Life Sciences [q-bio]/Microbiology and Parasitology/VirologyJournal articles %X BACKGROUND: Influenza A viruses are medically important viral pathogens that cause significant mortality and morbidity throughout the world. The recent emergence of a novel human influenza A virus (H1N1) poses a serious health threat. Molecular tests for rapid detection of this virus are urgently needed. METHODS: We developed a conventional 1-step RT-PCR assay and a 1-step quantitative real-time RT-PCR assay to detect the novel H1N1 virus, but not the seasonal H1N1 viruses. We also developed an additional real-time RT-PCR that can discriminate the novel H1N1 from other swine and human H1 subtype viruses. RESULTS: All of the assays had detection limits for the positive control in the range of 1.0 x 10(-4) to 2.0 x 10(-3) of the median tissue culture infective dose. Assay specificities were high, and for the conventional and real-time assays, all negative control samples were negative, including 7 human seasonal H1N1 viruses, 1 human H2N2 virus, 2 human seasonal H3N2 viruses, 1 human H5N1 virus, 7 avian influenza viruses (HA subtypes 4, 5, 7, 8, 9, and 10), and 48 nasopharyngeal aspirates (NPAs) from patients with noninfluenza respiratory diseases; for the assay that discriminates the novel H1N1 from other swine and human H1 subtype viruses, all negative controls were also negative, including 20 control NPAs, 2 seasonal human H1N1 viruses, 2 seasonal human H3N2 viruses, and 2 human H5N1 viruses. CONCLUSIONS: These assays appear useful for the rapid diagnosis of cases with the novel H1N1 virus, thereby allowing better pandemic preparedness. %G English %2 https://riip.hal.science/pasteur-00588929/document %2 https://riip.hal.science/pasteur-00588929/file/Poon_ClinChem_2009.pdf %L pasteur-00588929 %U https://riip.hal.science/pasteur-00588929 %~ RIIP %~ RIIP_HONGKONG