%0 Journal Article %T MicroRNA-101 targets MAPK phosphatase-1 to regulate the activation of MAPKs in macrophages. %+ Institut Pasteur de Shanghai, Académie des Sciences de Chine - Chinese Academy of Sciences (IPS-CAS) %+ Key Laboratory of Stem Cell Biology %A Zhu, Qing-Yuan %A Liu, Qin %A Chen, Jian-Xia %A Lan, Ke %A Ge, Bao-Xue %< avec comité de lecture %@ 0022-1767 %J Journal of Immunology %I Publisher : Baltimore : Williams & Wilkins, c1950-. Latest Publisher : Bethesda, MD : American Association of Immunologists %V 185 %N 12 %P 7435-42 %8 2010-12-15 %D 2010 %R 10.4049/jimmunol.1000798 %M 21068409 %Z Life Sciences [q-bio]/ImmunologyJournal articles %X MAPK phosphatase-1 (MKP-1) is an archetypical member of the dual-specificity phosphatase family that deactivates MAPKs. Induction of MKP-1 has been implicated in attenuating the LPS- or peptidoglycan-induced biosynthesis of proinflammatory cytokines, but the role of noncoding RNA in the expression of the MKP-1 is still poorly understood. In this study, we show that MKP-1 is a direct target of microRNA-101 (miR-101). Transfection of miR-101 attenuates induction of MKP-1 by LPS as well as prolonged activation of p38 and JNK/stress-activated protein kinase, whereas inhibition of miR-101 enhances the expression of MKP-1 and shortens p38 and JNK activation. We also found that expression of miR-101 is induced by multiple TLR ligands, including LPS, peptidoglycan, or polyinosinic-polycytidylic acid, and that inhibition of PI3K/Akt by LY294002 or Akt RNA interference blocks the induction of miR-101 by LPS in RAW264.7 macrophage cells. Moreover, treatment of cells with dexamethasone, a widely used anti-inflammatory agent, markedly inhibits miR-101 expression and enhances the expression of MKP-1 in LPS-stimulated macrophages. Together, these results indicate that miR-101 regulates the innate immune responses of macrophages to LPS through targeting MKP-1. %G English %2 https://riip.hal.science/pasteur-00624971/document %2 https://riip.hal.science/pasteur-00624971/file/JI-ae_ae_ae_i_GBXi_-2010.pdf %L pasteur-00624971 %U https://riip.hal.science/pasteur-00624971 %~ RIIP