%0 Journal Article %T A small RNA promotes siderophore production through transcriptional and metabolic remodeling. %+ Department of Biochemistry %+ Department of Microbiology %+ Institut Armand Frappier (INRS-IAF) %A Salvail, Hubert %A Lanthier-Bourbonnais, Pascale %A Sobota, Jason Michael %A Caza, Mélissa %A Benjamin, Julie-Anna M %A Mendieta, Martha Eugènia Sequeira %A Lépine, François %A Dozois, Charles M %A Imlay, James %A Massé, Eric %Z This work was funded by an operating grant to E. M. from the Natural Science and Engineering Research of Canada and to C.M.D. from Natural Science and Engineering Research of Canada and the Canada Research Chairs program. E.M. is a Canadian Institutes for Health Research New Investigator scholar. H.S. holds PhD fellowships from the Fonds Québécois de la Recherche sur la Nature et les Technologiques and Natural Science and Engineering Research of Canada. J.-A.M.B. holds a Natural Science and Engineering Research of Canada MSc fellowship. %< avec comité de lecture %@ 0027-8424 %J Proceedings of the National Academy of Sciences of the United States of America %I National Academy of Sciences %V 107 %N 34 %P 15223-8 %8 2010-08-24 %D 2010 %R 10.1073/pnas.1007805107 %M 20696910 %Z Life Sciences [q-bio]/Microbiology and Parasitology/BacteriologyJournal articles %X Siderophores are essential factors for iron (Fe) acquisition in bacteria during colonization and infection of eukaryotic hosts, which restrain iron access through iron-binding protein, such as lactoferrin and transferrin. The synthesis of siderophores by Escherichia coli is considered to be fully regulated at the transcriptional level by the Fe-responsive transcriptional repressor Fur. Here we characterized two different pathways that promote the production of the siderophore enterobactin via the action of the small RNA RyhB. First, RyhB is required for normal expression of an important enterobactin biosynthesis polycistron, entCEBAH. Second, RyhB directly represses the translation of cysE, which encodes a serine acetyltransferase that uses serine as a substrate for cysteine biosynthesis. Reduction of CysE activity by RyhB allows serine to be used as building blocks for enterobactin synthesis through the nonribosomal peptide synthesis pathway. Thus, RyhB plays an essential role in siderophore production and may modulate bacterial virulence through optimization of siderophore production. %G English %L pasteur-00720788 %U https://riip.hal.science/pasteur-00720788 %~ RIIP %~ INRS-IAF