%0 Journal Article %T Use of serum and blood samples on filter paper to improve the surveillance of dengue in Pacific Island Countries. %+ Institut Louis Malardé [Papeete] (ILM) %+ Université de la Polynésie Française (UPF) %+ Institut Pasteur de Nouvelle-Calédonie %+ Queensland University of Technology [Brisbane] (QUT) %+ Yap State Department of Health Service %+ Majuro Hospital %+ Secretariat of the Pacific Community %+ World Health Organization %+ Research Department %+ Pacific Technical Support Division %A Aubry, Maite %A Roche, Claudine %A Dupont-Rouzeyrol, Myrielle %A Aaskov, John %A Viallon, Jérôme %A Marfel, Maria %A Lalita, Paul %A Elbourne-Duituturaga, Salanieta %A Chanteau, Suzanne %A Musso, Didier %A Pavlin, Boris I %A Harrison, Dustin %A Kool, Jacob L %A Cao-Lormeau, Van-Mai %Z This work was supported by the "Fonds de Coopération Economique, Sociale et Culturelle pour le Pacifique" - Min- istère des Affaires Etrangères et Européennes, France (Convention No. 75/1/2009) (URL: http://www.diplomatie.gouv.fr) and by the "Agence Nationale pour la Recherche", France (Décisions attribu- tives d'aide N ANR-09-MIEN-028-01, ANR-09-MIEN-028-02), URL: http://www.agence-nationale-recherche.fr. The funders had no role in study design, data collection and analysis, decision to pub- lish, or preparation of the manuscript. %< avec comité de lecture %@ 1386-6532 %J Journal of Clinical Virology %I Elsevier %V 55 %N 1 %P 23-9 %8 2012-09 %D 2012 %R 10.1016/j.jcv.2012.05.010 %M 22695001 %K Dengue virus %K Filter paper %K Real-time RT-PCR Pacific Island Countries %K Surveillance Real-time RT-PCR Pacific Island Countries Surveillance %Z Life Sciences [q-bio]/Biochemistry, Molecular BiologyJournal articles %X BACKGROUND: In Pacific Island Countries (PICs) the epidemiology of dengue is characterized by long-term transmission of a single dengue virus (DENV) serotype. The emergence of a new serotype in one island country often indicates major outbreaks with this serotype will follow in other PICs. OBJECTIVES: Filter paper (FP) cards on which whole blood or serum from dengue suspected patients had been dried was evaluated as a method for transportation of this material by standard mail delivery throughout the Pacific. STUDY DESIGN: Twenty-two FP-dried whole blood samples collected from patients in New Caledonia and Wallis & Futuna Islands, during DENV-1 and DENV-4 transmission, and 76 FP-dried sera collected from patients in Yap State, Majuro (Republic of Marshall Islands), Tonga and Fiji, before and during outbreaks of DENV-2 in Yap State and DENV-4 in Majuro, were tested for the presence of DENV RNA, by serotype specific RT-PCR, at the Institut Louis Malardé in French Polynesia. RESULTS: The serotype of DENV could be determined, by a variety of RT-PCR procedures, in the FP-dried samples after more than three weeks of transport at ambient temperatures. In most cases, the sequencing of the envelope gene to genotype the viruses also was possible. CONCLUSIONS: The serotype and genotype of DENV can be determined from FP-dried serum or whole blood samples transported over thousands of kilometers at ambient, tropical, temperatures. This simple and low-cost approach to virus identification should be evaluated in isolated and resource poor settings for surveillance for a range of significant viral diseases. %G English %2 https://riip.hal.science/pasteur-00734957/document %2 https://riip.hal.science/pasteur-00734957/file/1-s2.0-S138665321200203X-main.pdf %L pasteur-00734957 %U https://riip.hal.science/pasteur-00734957 %~ IRD %~ RIIP %~ RIIP_NOUVELLECALEDONIE %~ UNIV-POLYNESIE %~ UPF