%0 Journal Article %T Biosurfactant production by halotolerant Rhodococcus fascians from Casey Station, Wilkes Land, Antarctica. %+ Institut Stephan Angeloff %+ Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH / Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures (DSMZ) %A Gesheva, Victoria %A Stackebrandt, Erko %A Vasileva-Tonkova, Evgenia %< avec comité de lecture %@ 0343-8651 %J Current Microbiology %I Springer Verlag %V 61 %N 2 %P 112-7 %8 2010-08 %D 2010 %R 10.1007/s00284-010-9584-7 %M 20135319 %Z Life Sciences [q-bio]/Microbiology and ParasitologyJournal articles %X Isolate A-3 from Antarctic soil in Casey Station, Wilkes Land, was characterized for growth on hydrocarbons. Use of glucose or kerosene as a sole carbon source in the culture medium favoured biosynthesis of surfactant which, by thin-layer chromatography, indicated the formation of a rhamnose-containing glycolipid. This compound lowered the surface tension at the air/water interface to 27 mN/m as well as inhibited the growth of B. subtilis ATCC 6633 and exhibited hemolytic activity. A highly hydrophobic surface of the cells suggests that uptake occurs via a direct cell-hydrocarbon substrate contact. Strain A-3 is Gram-positive, halotolerant, catalase positive, urease negative and has rod-coccus shape. Its cell walls contained meso-diaminopimelic acid. Phylogenetic analysis based on comparative analysis of 16S rRNA gene sequences revealed that strain A-3 is closely related to Rhodococcus fascians with which it shares 100% sequence similarity. This is the first report on rhamnose-containing biosurfactant production by Rhodococcus fascians isolated from Antarctic soil. %G English %2 https://riip.hal.science/pasteur-00748956/document %2 https://riip.hal.science/pasteur-00748956/file/Gesheva_et_al_Curr_Microbiol_2010.pdf %L pasteur-00748956 %U https://riip.hal.science/pasteur-00748956 %~ RIIP %~ RIIP_SA