%0 Journal Article %T Upregulation of connexins 30 and 32 gap junctions in rat hippocampus at transcription level by chronic central injection of lipopolysaccharide. %+ Institut Pasteur d'Iran %+ Department of Physiology %+ Department of Physiology %A Abbasian, Mohammad %A Sayyah, Mohammad %A Babapour, Vahab %A Mahdian, Reza %A Choopani, Samira %A Kaviani, Bahar %Z Financial support of Pasteur Institute of Iran (grant No. 472) is acknowledged. %< avec comité de lecture %@ 1028-852X %J Iranian Biomedical Journal %I Pasteur Institute of Iran %V 16 %N 3 %P 127-32 %8 2012-07 %D 2012 %R 10.6091/ibj.1099.2012 %M 23023213 %K Connexin 30 %K Connexin 32 %K Hippocampus %Z Life Sciences [q-bio]/CancerJournal articles %X Background: Gap junctions composed of connexins (Cx) are functional in cell defense by propagation of toxic/death molecules to neighboring cells. Hippocampus, one of the brain regions with particular vulnerability to damage, has a wide network of gap junctions. Functional response of astrocytic Cx30 and neuronal Cx32 to hippocampal damage is unknown. Methods: We infused lipopolysaccharide (LPS) intracerebroventricularly (2.5 mug/rat) once daily for two weeks to create neuroinflammation. The mRNA and protein levels of the Cx were measured in the hippocampus after 1st, 7th and 14th injection by real-time PCR and Western-blot techniques. Results: A significant increase in Cx32 and Cx30 gene expression was observed after 7th and 14th injection of LPS with no significant change in their protein abundance. Conclusion: Transcriptional overexpression of hippocampal Cx30 and Cx32 could be an adaptive response to production of intracellular toxic molecules but it is not accompanied with post- transcriptional overexpression and might have no functional impact. %G English %Z Mohammad Abbasian %Z Mohammad Sayyah %Z Vahab Babapour %Z Reza Mahdian %Z Samira Choopani %Z Bahar Kaviani %2 https://riip.hal.science/pasteur-00754040/document %2 https://riip.hal.science/pasteur-00754040/file/Iranian_Biomedical_Journal_-v16n3p132.pdf %L pasteur-00754040 %U https://riip.hal.science/pasteur-00754040 %~ RIIP %~ RIIP_IRAN