%0 Journal Article %T Expression, purification, crystallization and preliminary crystallographic studies of cis-biphenyl-2,3-dihydrodiol-2,3-dehydrogenase from Pandoraea pnomenusa B-356. %+ Department of Biotechnology [Roorkee) %+ Institut Armand Frappier (INRS-IAF) %A Sakshi, ? %A Patil, Dipak N %A Tomar, Shailly %A Sylvestre, Michel %A Kumar, Pravindra %< avec comité de lecture %@ 1744-3091 %J Acta Crystallographica Section F: Structural Biology and Crystallization Communications %I International Union of Crystallography %V 66 %N Pt 11 %P 1517-20 %8 2010-11-01 %D 2010 %R 10.1107/S1744309110036894 %M 21045310 %K cis-biphenyl-2 %K 3-dihydrodiol-2 %K 3-dehydrogenase %K Pandoraea pnomenusa %K biodegradation %Z Life Sciences [q-bio]/Biotechnology %Z Computer Science [cs]/BiotechnologyJournal articles %X cis-Biphenyl-2,3-dihydrodiol-2,3-dehydrogenase (BphB) is involved in the aerobic biodegradation of biphenyl and polychlorinated biphenyls. BphB from Pandoraea pnomenusa strain B-356 was overexpressed in Escherichia coli, purified to homogeneity and crystallized. Crystals were obtained by the sitting-drop vapour-diffusion method using polyethylene glycol 3350 and 0.2 M sodium malonate. A BphB crystal diffracted to 2.8 Å resolution and belonged to space group P4(3)2(1)2, with unit-cell parameters a = b = 75.2, c = 180.4 Å. Preliminary crystallographic analysis indicated the presence of two molecules in the asymmetric unit, giving a Matthews coefficient of 2.2 Å(3) Da(-1) and a solvent content of 44%. %G English %L pasteur-00819542 %U https://riip.hal.science/pasteur-00819542 %~ RIIP %~ INRS-IAF