%0 Journal Article %T Structure, properties and applications of rhamnolipids produced by Pseudomonas aeruginosa L2-1 from cassava wastewater %+ Universidade Estadual Paulista Júlio de Mesquita Filho = São Paulo State University (UNESP) %+ Universidade de São Paulo = University of São Paulo (USP) %+ Institut Armand Frappier (INRS-IAF) %A Costa, Siddhartha G.V.A.O. %A Nitschke, Marcia %A Lépine, Francois %A Déziel, Eric %A Contiero, Jonas %Z We thank Fundação de Amparo a Pesquisa do Estado de São Paulo (FAPESP) for fellowships and financial supports. %< avec comité de lecture %@ 1359-5113 %J Process Biochemistry %I Elsevier %V 45 %N 9 %P 1511-1516 %8 2010 %D 2010 %R 10.1016/j.procbio.2010.05.033 %K Rhamnolipids %K P. aeruginosa %K Cassava wastewater %K Tensioactive properties %K Oil removal %Z Life Sciences [q-bio]/Biotechnology %Z Computer Science [cs]/BiotechnologyJournal articles %X The properties and applications of rhamnolipid surfactants produced by Pseudomonas aeruginosa L2-1 from cassava wastewater added with waste cooking oil (CWO) as low-cost substrate, were investigated and compared with the commercial rhamnolipid mixture JBR599 (Jeneil Biosurfactant Co., Saukville, USA). The rhamnolipids produced by strain L2-1 were characterized by high performance liquid chromatography-mass spectrometry. Sixteen different rhamnolipid congeners were detected, with Rha-C10-C10 and Rha-Rha-C10-C10 being the most abundant. The L2-1 rhamnolipids from CWO showed similar or better tensioactive properties than those from JBR599, with a minimal surface tension of 30 mN/m and a critical micelle concentration (CMC) of 30 mg/l. The L2-1 biosurfactants formed stable emulsions with several hydrocarbons and showed excellent emulsification of soybean oil (100%). These rhamnolipids removed 69% of crude oil present in contaminated sand samples at the CMC and presented antimicrobial activity against Bacillus cereus (32 μg/ml), Micrococcus luteus (32 μg/ml) and Staphylococcus aureus (128 μg/ml). These results demonstrate that the rhamnolipids produced in CWO can be useful for industrial applications, such as the bioremediation of oil spills. %G English %L pasteur-00819599 %U https://riip.hal.science/pasteur-00819599 %~ RIIP %~ INRS-IAF