%0 Journal Article %T Ini1/hSNF5 is dispensable for retrovirus-induced cytoplasmic accumulation of PML and does not interfere with integration. %+ Biologie Cellulaire du Noyau (BCN) %+ Expression Génétique et Maladies (EGM) %A Boese, Annette %A Sommer, Peter %A Gaussin, Armelle %A Reimann, Andreas %A Nehrbass, Ulf %< avec comité de lecture %@ 0014-5793 %J FEBS Letters %I Wiley %V 578 %N 3 %P 291-6 %8 2004-12-17 %D 2004 %R 10.1016/j.febslet.2004.11.016 %M 15589835 %Z Life Sciences [q-bio]/Microbiology and Parasitology/VirologyJournal articles %X Retroviral infection triggers the cytoplasmic translocation of two Crm1-dependent shuttle factors, namely the Ini1 (integrase interactor 1, hSNF5) and the promyelocytic leukemia (PML) protein. Blocking nuclear export of shuttle factors by leptomycin B increases the efficiency of retroviral integration, suggesting that some may mediate antiviral activity. While PML was shown to counteract proviral establishment, it remained unclear whether Ini1, a protein implicated in various processes during human immunodeficiency virus replication, has the same potential. Employing RNA interference-mediated knock-down of Ini1, we show here that the simultaneous accumulation of both proteins in the cytoplasm likely reflects two non-interdependent phenomena. Furthermore, Ini1 does not interfere with retroviral integration, as cells lacking Ini1 show no increased infection susceptibility. %G English %2 https://riip.hal.science/pasteur-00850261/document %2 https://riip.hal.science/pasteur-00850261/file/BOESE2004.pdf %L pasteur-00850261 %U https://riip.hal.science/pasteur-00850261 %~ PASTEUR %~ RIIP %~ CNRS %~ RIIP_PARIS