%0 Journal Article %T H4K16 acetylation affects recombination and ncRNA transcription at rDNA in Saccharomyces cerevisiae. %+ Department of Biology and Biotechnology "Charles Darwin" %+ National Research Council of Italy | Consiglio Nazionale delle Ricerche (CNR) %A Cesarini, Elisa %A d'Alfonso, Anna %A Camilloni, Giorgio %< avec comité de lecture %J Molecular Biology of the Cell %I American Society for Cell Biology %V 23 %N 14 %P 2770-81 %8 2012-07 %D 2012 %R 10.1091/mbc.E12-02-0095 %M 22621897 %Z Life Sciences [q-bio]/Biochemistry, Molecular BiologyJournal articles %X Transcription-associated recombination is an important process involved in several aspects of cell physiology. In the ribosomal DNA (rDNA) of Saccharomyces cerevisiae, RNA polymerase II transcription-dependent recombination has been demonstrated among the repeated units. In this study, we investigate the mechanisms controlling this process at the chromatin level. On the basis of a small biased screening, we found that mutants of histone deacetylases and chromatin architectural proteins alter both the amount of Pol II-dependent noncoding transcripts and recombination products at rDNA in a coordinated manner. Of interest, chromatin immunoprecipitation analyses in these mutants revealed a corresponding variation of the histone H4 acetylation along the rDNA repeat, particularly at Lys-16. Here we provide evidence that a single, rapid, and reversible posttranslational modification-the acetylation of the H4K16 residue-is involved in the coordination of transcription and recombination at rDNA. %G English %2 https://riip.hal.science/pasteur-00952222/document %2 https://riip.hal.science/pasteur-00952222/file/Cesarinietal2012.pdf %L pasteur-00952222 %U https://riip.hal.science/pasteur-00952222 %~ RIIP %~ RIIP_FCB