%0 Journal Article %T Culture of Borrelia persica and its flagellar antigen in vitro. %+ Department of Biochemistry %+ Department of Parasitology %+ Department of Immunology %A Zamani, Zahra %A Arjmand, Mohammad %A Oreiz, Farzad %A Soleimani, Mohammad %A Hosseini, Seyed Hossein %A Assmar, Mehdi %A Javadian, Saifeddin %A Saghiri, Reza %A Pourfallah, Fatemeh %Z Institute Pasteur of Iran %< avec comité de lecture %@ 1028-8880 %J Pakistan Journal of Biological Sciences %I Asian Network for Scientific Information (ANSINET) %V 17 %N 2 %P 190-7 %8 2014-01-15 %D 2014 %R 10.3923/pjbs.2014.190.197 %M 24783801 %K Borrelia persica %K relapsing fever %K indirect haemmaglutination kit %Z Life Sciences [q-bio]/Microbiology and ParasitologyJournal articles %X Borrelia persica is a strain seen only in the Middle East and responsible for relapsing fever. These spirochetes are notable for multiphasic antigenic variation of polymorphic outer membrane lipoproteins, a phenomenon responsible for immune evasion. Diagnosis of the disease is a problem and requires a fixed antigen like the flagellar antigen. In vitro culture of B. persica was carried out for the first time and flagellar antigen was purified from culture. 10% SDS was added to the mixture to dissolve the cell wall and then the solution was sheared in an Omni mixer. Electron microscopy confirmed the purity of a 42 KDa periplasmic antigen as revealed by SDS-PAGE. Indirect haemagglutination kits were designed using the pure flagella and tested for cross reactivity with another relapsing fever spirochaete Borrelia microtii positive serum. The kit showed 98% sensitivity and 95% specificity. %G English %2 https://riip.hal.science/pasteur-01057589/document %2 https://riip.hal.science/pasteur-01057589/file/B.persicaq.pdf %L pasteur-01057589 %U https://riip.hal.science/pasteur-01057589 %~ RIIP %~ RIIP_IRAN