%0 Journal Article %T Role of alpha-synuclein in autophagy modulation of primary human T lymphocytes. %+ Department of Cell Biology and Neurosciences %+ Department of Medical Sciences %+ Department of Internal Medicine, Lupus Clinic %+ Department of Medical Sciences %+ Department of Biology and Biotechnology "Charles Darwin" %+ Department of Technology and Health %+ Department of Oncology and Hematology, Humanitas Cancer Center %+ Department of Medical Biotechnology and Translational Medicine %+ Laboratory of Clinical and Behavioral Neurology %+ Movement Disorder Unit, Sant’Andrea Hospital %+ Department of Therapeutic Research and Medicine Evaluation %A Colasanti, T %A Vomero, M %A Alessandri, C %A Barbati, C %A Maselli, A %A Camperio, C %A Conti, F %A Tinari, A %A Carlo-Stella, C %A Tuosto, L %A Benincasa, D %A Valesini, G %A Malorni, W %A Pierdominici, M %A Ortona, E %Z This study was partially supported by grants from AIRC to WM (MCO – 9998 and 11505) and from the Italian Ministry of Health to EO (U7A).CC and LT are supported by Fondazione Italiana Sclerosi Multipla-FISM-Cod. 2011/R/36 (Genova, Italy). %< avec comité de lecture %@ 2041-4889 %J Cell Death and Disease %I Nature Publishing Group %V 5 %P e1265 %8 2014-05-29 %D 2014 %R 10.1038/cddis.2014.211 %M 24874737 %Z Life Sciences [q-bio]/Biochemistry, Molecular Biology %Z Life Sciences [q-bio]/ImmunologyJournal articles %X It has been demonstrated that α-synuclein can aggregate and contribute to the pathogenesis of some neurodegenerative diseases and it is capable of hindering autophagy in neuronal cells. Here, we investigated the implication of α-synuclein in the autophagy process in primary human T lymphocytes. We provide evidence that: (i) knocking down of the α-synuclein gene resulted in increased autophagy, (ii) autophagy induction by energy deprivation was associated with a significant decrease of α-synuclein levels, (iii) autophagy inhibition by 3-methyladenine or by ATG5 knocking down led to a significant increase of α-synuclein levels, and (iv) autophagy impairment, constitutive in T lymphocytes from patients with systemic lupus erythematosus, was associated with abnormal accumulation of α-synuclein aggregates. These results suggest that α-synuclein could be considered as an autophagy-related marker of peripheral blood lymphocytes, potentially suitable for use in the clinical practice. %G English %2 https://riip.hal.science/pasteur-01197320/document %2 https://riip.hal.science/pasteur-01197320/file/cddis2014211a.pdf %L pasteur-01197320 %U https://riip.hal.science/pasteur-01197320 %~ RIIP