%0 Journal Article %T Human multidrug-resistant Mycobacterium bovis infection in Mexico. %+ Escuela Nacional de Ciencias Bilogicas %+ Instituto de Diagnóstico y Referencia Epidemiológicos %+ Unité de la Tuberculose et des Mycobactéries - WHO Supranational TB Reference Laboratory %+ Laboratorio Estatal de Salud Pública %+ Centro Mesoamericano de Estudios en Salud Pública y Desastres %+ Escuela de Ciencias de la Salud %A Vazquez-Chacon, Carlos A %A Martínez-Guarneros, Armando %A Couvin, David %A González-Y-Merchand, Jorge A %A Rivera-Gutierrez, Sandra %A Escobar-Gutierrez, Alejandro %A De-La-Cruz López, Juan J %A Gomez-Bustamante, Adriana %A Gonzalez-Macal, Gabriela A %A Gonçalves Rossi, Livia Maria %A Muñiz-Salazar, Raquel %A Rastogi, Nalin %A Vaughan, Gilberto %Z This work was partially supported by CONACYT-SALUD-2010-C01-139579 and as part of the 7th Framework Program, European Commission, contract No. HEALTH-F3-2008-200999, and by grants CB-2010-156347, CONACYT and SIP20140970, IPN, Mexico. %< avec comité de lecture %@ 1472-9792 %J Tuberculosis %I Elsevier %V 95 %N 6 %P 802-9 %8 2015-11-30 %D 2015 %R 10.1016/j.tube.2015.07.010 %M 26299906 %Z Life Sciences [q-bio]/Microbiology and Parasitology/BacteriologyJournal articles %X Here, we describe the molecular characterization of six human Mycobacterium bovis clinical isolates, including three multidrug resistant (MDR) strains, collected in Mexico through the National Survey on Tuberculosis Drug Resistance (ENTB-2008), a nationally representative survey conducted during 2008-2009 in nine states with a stratified cluster sampling design. The genetic background of bovine M. bovis strains identified in three different states of Mexico was studied in parallel to assess molecular relatedness of bovine and human strains. Additionally, resistance to first and second line anti-tuberculosis (TB) drugs and molecular identification of mutations conferring drug resistance was also performed. All strains were characterized by spoligotyping and 24-loci MIRU-VNTRs, and analyzed using the SITVIT2 (n = 112,000 strains) and SITVITBovis (n = 25,000 strains) proprietary databases of Institut Pasteur de la Guadeloupe. Furthermore, data from this study (n = 55 isolates), were also compared with genotypes recorded for M. bovis from USA (n = 203), Argentina (n = 726), as well as other isolates from Mexico (independent from the present study; n = 147), to determine any evidence for genetic relatedness between circulating M. bovis strains. The results showed that all human M. bovis cases were not genetically related between them or to any bovine strain. Interestingly, a high degree of genetic variability was observed among bovine strains. Several autochthonous and presumably imported strains were identified. The emergence of drug-resistant M. bovis is an important public health problem that jeopardizes the success of TB control programs in the region. %G English %L pasteur-01304571 %U https://riip.hal.science/pasteur-01304571 %~ RIIP %~ RIIP_GUADELOUPE