%0 Journal Article %T HlyF Produced by Extraintestinal Pathogenic Escherichia coli Is a Virulence Factor That Regulates Outer Membrane Vesicle Biogenesis. %+ University of Miyazaki %+ Université de Toulouse (UT) %+ Institut Armand Frappier (INRS-IAF) %+ Infectiologie et Santé Publique (UMR ISP) %+ Department of Applied Chemistry %+ Centre Hospitalier Universitaire de Toulouse (CHU Toulouse) %A Murase, Kazunori %A Martin, Patricia %A Porcheron, Gaëlle %A Houle, Sébastien %A Helloin, Emmanuelle %A Pénary, Marie %A Nougayrède, Jean-Philippe %A Dozois, Charles M %A Hayashi, Tetsuya %A Oswald, Eric %Z This work was supported by the French government, through the Investments for the Future program (ANR-11-EQPX-0003); the Ministry of Education, Culture, Sports, Science, and Technology of Japan (Grants-in-Aid for Young [B] 26860293 to K. M.); the National Science and Engineering Research Council of Canada (Discovery grant to C. M. D.); and a Canada Research Chair (to C. M. D.). %< avec comité de lecture %@ 0022-1899 %J Journal of Infectious Diseases %I Oxford University Press (OUP) %V 213 %N 5 %P 856-65 %8 2016-03-01 %D 2016 %R 10.1093/infdis/jiv506 %M 26494774 %K HlyF %K OMV %K pathogenesis %Z Life Sciences [q-bio] %Z Life Sciences [q-bio]/Microbiology and Parasitology/BacteriologyJournal articles %X Escherichia coli can cause extraintestinal infections in humans and animals. The hlyF gene is epidemiologically associated with virulent strains of avian pathogenic E. coli and human neonatal meningitis-associated E. coli. We demonstrated that culture supernatants of E. coli expressing HlyF induced autophagy in eukaryotic cells. This phenotype coincided with an enhanced production of outer membrane vesicles (OMVs) by bacteria expressing HlyF. The HlyF protein displays a predicted catalytic domain of the short-chain dehydrogenase/reductase superfamily. This conserved domain was involved the ability of HlyF to promote the production of OMVs. The increased production of OMVs was associated with the release of toxins. hlyF was shown to be expressed during extraintestinal infection and to play a role in the virulence of extraintestinal pathogenic E. coli in a chicken model of colibacillosis. This is the first evidence that pathogenic bacteria produce a virulence factor directly involved in the production of OMVs. %G English %L pasteur-01351505 %U https://riip.hal.science/pasteur-01351505 %~ INSERM %~ RIIP %~ UNIV-TOURS %~ INRA %~ INRS-IAF %~ AGREENIUM %~ INRAE %~ ANR %~ INFECTIOLOGIE-SANTE-PUBLIQUE %~ ISP-CIRM %~ INRAEVALDELOIRE