%0 Journal Article %T Regulation of viral gene expression by the herpes simplex virus 1UL24 protein (HSV-1UL24 inhibits accumulation of viral transcripts). %+ Institut Armand Frappier (INRS-IAF) %+ Texas Biomedical Research Institute [San Antonio, TX] %+ Centre de Recherche du Centre Hospitalier de l’Université de Montréal (CR CHUM) %A Sanabria-Solano, Carolina %A Gonzalez, Carmen Elena %A Richerioux, Nicolas %A Bertrand, Luc %A Dridi, Slimane %A Griffiths, Anthony %A Langelier, Yves %A Pearson, Angela %Z CSS received a scholarship from the Foundation Armand-Frappier. LB received scholarships from the Foundation Armand-Frappier and the Canadian Institutes of Health Research (CIHR). This work was supported by Institutional funds to AG, and by operating grants from CIHR to YL (MOP 67052) and AP (MOP 82924). This work was also supported by an infrastructure grant from the Canada Foundation for Innovation (9991) to AP. %< avec comité de lecture %@ 0042-6822 %J Virology %I Elsevier %V 495 %P 148-60 %8 2016-07-31 %D 2016 %R 10.1016/j.virol.2016.05.006 %M 27214229 %K Herpes simplex virus 1 %K UL24 %K Viral gene regulation %Z Life Sciences [q-bio]Journal articles %X UL24 is conserved among all Herpesviridae. In herpes simplex virus 1 (HSV-1), UL24 mutations lead to reduced viral titers both in cell culture and in vivo, and reduced pathogenicity. The human cytomegalovirus ortholog of UL24 has a gene regulatory function; however, it is not known whether other UL24 orthologs also affect gene expression. We discovered that in co-transfection experiments, expression of UL24 correlated with a reduction in the expression of several viral proteins and transcripts. Substitution mutations targeting conserved residues in UL24 impaired this function. Reduced transcript levels did not appear attributable to changes in mRNA stability. The UL24 ortholog of Herpes B virus exhibited a similar activity. An HSV-1 mutant that does not express UL24 produced more viral R1 and R2 transcripts than the wild type or rescue virus relative to the amount of viral DNA. These results reveal a new role for HSV-1UL24 in regulating viral mRNA accumulation. %G English %L pasteur-01351545 %U https://riip.hal.science/pasteur-01351545 %~ RIIP %~ INRS-IAF