Spectral phasor analysis of LAURDAN fluorescence in live A549 lung cells to study the hydration and time evolution of intracellular lamellar body-like structures. - RIIP - Réseau International des Instituts Pasteur Access content directly
Journal Articles BBA - Biochimica et Biophysica Acta Year : 2016

Spectral phasor analysis of LAURDAN fluorescence in live A549 lung cells to study the hydration and time evolution of intracellular lamellar body-like structures.

Abstract

Using LAURDAN spectral imaging and spectral phasor analysis we concurrently studied the growth and hydration state of subcellular organelles (lamellar body-like, LB-like) from live A549 lung cancer cells at different post-confluence days. Our results reveal a time dependent two-step process governing the size and hydration of these intracellular LB-like structures. Specifically, a first step (days 1 to 7) is characterized by an increase in their size, followed by a second one (days 7 to 14) where the organelles display a decrease in their global hydration properties. Interestingly, our results also show that their hydration properties significantly differ from those observed in well-characterized artificial lamellar model membranes, challenging the notion that a pure lamellar membrane organization is present in these organelles at intracellular conditions. Finally, these LB-like structures show a significant increase in their hydration state upon secretion, suggesting a relevant role of entropy during this process.

Dates and versions

pasteur-01498359 , version 1 (09-10-2017)

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Leonel Malacrida, Soledad Astrada, Arturo Briva, Mariela Bollati-Fogolín, Enrico Gratton, et al.. Spectral phasor analysis of LAURDAN fluorescence in live A549 lung cells to study the hydration and time evolution of intracellular lamellar body-like structures.. BBA - Biochimica et Biophysica Acta, 2016, 1858 (11), pp.2625-2635. ⟨10.1016/j.bbamem.2016.07.017⟩. ⟨pasteur-01498359⟩
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