<?xml version="1.0" encoding="utf-8"?>
<TEI xmlns="http://www.tei-c.org/ns/1.0" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:hal="http://hal.archives-ouvertes.fr/" xmlns:gml="http://www.opengis.net/gml/3.3/" xmlns:gmlce="http://www.opengis.net/gml/3.3/ce" version="1.1" xsi:schemaLocation="http://www.tei-c.org/ns/1.0 http://api.archives-ouvertes.fr/documents/aofr-sword.xsd">
  <teiHeader>
    <fileDesc>
      <titleStmt>
        <title>HAL TEI export of pasteur-01534474</title>
      </titleStmt>
      <publicationStmt>
        <distributor>CCSD</distributor>
        <availability status="restricted">
          <licence target="https://creativecommons.org/publicdomain/zero/1.0/">CC0 1.0 - Universal</licence>
        </availability>
        <date when="2026-05-03T05:14:07+02:00"/>
      </publicationStmt>
      <sourceDesc>
        <p part="N">HAL API Platform</p>
      </sourceDesc>
    </fileDesc>
  </teiHeader>
  <text>
    <body>
      <listBibl>
        <biblFull>
          <titleStmt>
            <title xml:lang="en">The SAT protein of porcine parvovirus accelerates viral spreading through irreversible ER stress induction.</title>
            <author role="crp">
              <persName>
                <forename type="first">István</forename>
                <surname>Mészáros</surname>
              </persName>
              <email type="md5">98f0dc86c1d697c7ba5c56acf03514fb</email>
              <email type="domain">agrar.mta.hu</email>
              <idno type="idhal" notation="numeric">1010950</idno>
              <idno type="halauthorid" notation="string">1187308-1010950</idno>
              <affiliation ref="#struct-306576"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Renáta</forename>
                <surname>Tóth</surname>
              </persName>
              <idno type="halauthorid">1178395-0</idno>
              <affiliation ref="#struct-306576"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Ferenc</forename>
                <surname>Olasz</surname>
              </persName>
              <idno type="halauthorid">1178396-0</idno>
              <affiliation ref="#struct-306576"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Peter</forename>
                <surname>Tijssen</surname>
              </persName>
              <idno type="halauthorid">652421-0</idno>
              <affiliation ref="#struct-54664"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Zoltán</forename>
                <surname>Zádori</surname>
              </persName>
              <idno type="halauthorid">1178397-0</idno>
              <affiliation ref="#struct-306576"/>
            </author>
            <editor role="depositor">
              <persName>
                <forename>Michel</forename>
                <surname>Courcelles</surname>
              </persName>
              <email type="md5">235ee71abfb5f3fb19e1b204192e01ee</email>
              <email type="domain">iaf.inrs.ca</email>
            </editor>
            <funder>The study was supported by the  Hungarian Scientific Research Fund (K108607) and National 575 Research, Development and Innovation Office (K119381)</funder>
          </titleStmt>
          <editionStmt>
            <edition n="v1" type="current">
              <date type="whenSubmitted">2017-06-07 16:05:15</date>
              <date type="whenModified">2024-12-19 11:12:04</date>
              <date type="whenReleased">2017-06-26 10:42:38</date>
              <date type="whenProduced">2017-05-31</date>
              <date type="whenEndEmbargoed">2017-12-26</date>
              <ref type="file" target="https://riip.hal.science/pasteur-01534474v1/document">
                <date notBefore="2017-12-26"/>
              </ref>
              <ref type="file" subtype="publisherPaid" n="1" target="https://riip.hal.science/pasteur-01534474v1/file/J.%20Virol.-2017-Me%CC%81sza%CC%81ros-JVI.00627-17.pdf" id="file-1534474-1603451">
                <date notBefore="2017-12-26"/>
              </ref>
              <ref type="externalLink" target="https://jvi.asm.org/content/jvi/91/16/e00627-17.full.pdf"/>
            </edition>
            <respStmt>
              <resp>contributor</resp>
              <name key="157387">
                <persName>
                  <forename>Michel</forename>
                  <surname>Courcelles</surname>
                </persName>
                <email type="md5">235ee71abfb5f3fb19e1b204192e01ee</email>
                <email type="domain">iaf.inrs.ca</email>
              </name>
            </respStmt>
          </editionStmt>
          <publicationStmt>
            <distributor>CCSD</distributor>
            <idno type="halId">pasteur-01534474</idno>
            <idno type="halUri">https://riip.hal.science/pasteur-01534474</idno>
            <idno type="halBibtex">meszaros:pasteur-01534474</idno>
            <idno type="halRefHtml">&lt;i&gt;Journal of Virology&lt;/i&gt;, 2017, &lt;a target="_blank" href="https://dx.doi.org/10.1128/jvi.00627-17"&gt;&amp;#x27E8;10.1128/jvi.00627-17&amp;#x27E9;&lt;/a&gt;</idno>
            <idno type="halRef">Journal of Virology, 2017, &amp;#x27E8;10.1128/jvi.00627-17&amp;#x27E9;</idno>
            <availability status="restricted">
              <licence target="https://about.hal.science/hal-authorisation-v1/">HAL Authorization<ref corresp="#file-1534474-1603451"/></licence>
            </availability>
          </publicationStmt>
          <seriesStmt>
            <idno type="stamp" n="RIIP">Pasteur Network</idno>
            <idno type="stamp" n="INRS-IAF" corresp="RIIP">Institut Armand Frappier</idno>
          </seriesStmt>
          <notesStmt>
            <note type="audience" n="2">International</note>
            <note type="popular" n="0">No</note>
            <note type="peer" n="1">Yes</note>
          </notesStmt>
          <sourceDesc>
            <biblStruct>
              <analytic>
                <title xml:lang="en">The SAT protein of porcine parvovirus accelerates viral spreading through irreversible ER stress induction.</title>
                <author role="crp">
                  <persName>
                    <forename type="first">István</forename>
                    <surname>Mészáros</surname>
                  </persName>
                  <email type="md5">98f0dc86c1d697c7ba5c56acf03514fb</email>
                  <email type="domain">agrar.mta.hu</email>
                  <idno type="idhal" notation="numeric">1010950</idno>
                  <idno type="halauthorid" notation="string">1187308-1010950</idno>
                  <affiliation ref="#struct-306576"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Renáta</forename>
                    <surname>Tóth</surname>
                  </persName>
                  <idno type="halauthorid">1178395-0</idno>
                  <affiliation ref="#struct-306576"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Ferenc</forename>
                    <surname>Olasz</surname>
                  </persName>
                  <idno type="halauthorid">1178396-0</idno>
                  <affiliation ref="#struct-306576"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Peter</forename>
                    <surname>Tijssen</surname>
                  </persName>
                  <idno type="halauthorid">652421-0</idno>
                  <affiliation ref="#struct-54664"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Zoltán</forename>
                    <surname>Zádori</surname>
                  </persName>
                  <idno type="halauthorid">1178397-0</idno>
                  <affiliation ref="#struct-306576"/>
                </author>
              </analytic>
              <monogr>
                <idno type="halJournalId" status="VALID">6614</idno>
                <idno type="issn">0022-538X</idno>
                <idno type="eissn">1098-5514</idno>
                <title level="j">Journal of Virology</title>
                <imprint>
                  <publisher>American Society for Microbiology</publisher>
                  <date type="datePub">2017-05-31</date>
                </imprint>
              </monogr>
              <idno type="doi">10.1128/jvi.00627-17</idno>
              <idno type="pubmed">28566374</idno>
            </biblStruct>
          </sourceDesc>
          <profileDesc>
            <langUsage>
              <language ident="en">English</language>
            </langUsage>
            <textClass>
              <classCode scheme="halDomain" n="sdv">Life Sciences [q-bio]</classCode>
              <classCode scheme="halDomain" n="sdv.mp.vir">Life Sciences [q-bio]/Microbiology and Parasitology/Virology</classCode>
              <classCode scheme="halTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halOldTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halTreeTypology" n="ART">Journal articles</classCode>
            </textClass>
            <abstract xml:lang="en">
              <p>The SAT protein of porcine parvovirus (PPV) accumulates in the endoplasmic reticulum (ER) and SAT deletion induces "slow spreading" phenotype. The in vitro comparison of the wild type Kresse strain and its SAT(-) knockout mutant revealed that prolonged cell integrity and late viral release are responsible for the slower spreading of the SAT(-) virus. During PPV infection, regardless of the presence or absence of SATp, the expression of downstream ER stress response proteins (Xbp1 and CHOP) was induced. However, in the absence of SATp, significant differences were detected in the quantity and the localization of CHOP, suggesting a role of SATp in the induction of irreversible ER stress in infected cells. The involvement of irreversible ER stress induction in PT cell necrosis and the viral egress was confirmed by treatment of infected cells by ER stress inducing chemicals (MG132, DTT and Thapsigargin) that accelerated the egress and spreading both the wild type and the SAT(-) viruses. UV stress induction had no beneficial effect to PPV infectionunderscoring the specificity of ER stress pathways in the process. However, induction of CHOP and its nuclear translocation cannot alone be responsible for the biological effect of SAT, since nuclear CHOP could not complement the lack of SAT in a co-expression experiment.IMPORTANCE SATp is encoded in an alternative ORF of the PPV genome. Earlier we showed that SATp of the attenuated PPV-NADL-2 strain accumulates in the ER and accelerates virus release and spreading. Our present work revealed that "slow spreading" is a general feature of the SAT(-) PPV viruses and is the consequence of prolonged cell integrity. PPV infection induced ER stress in the infected cells regardless of SATp presence, as demonstrated by the morphological changes of the ER, and expression of the stress response proteins XBP1 and CHOP. However, the presence of SATp made the ER stress more severe and accelerated the cell death during infection as shown by the higher expression rate and the alteration of the localization of CHOP. The beneficial effect of irreversible ER stress on PPV spread was confirmed by the treatment of the infected cells with ER stress inducing chemicals.</p>
            </abstract>
          </profileDesc>
        </biblFull>
      </listBibl>
    </body>
    <back>
      <listOrg type="structures">
        <org type="institution" xml:id="struct-306576" status="VALID">
          <idno type="ROR">https://ror.org/02ks8qq67</idno>
          <orgName>Hungarian Academy of Sciences</orgName>
          <orgName type="acronym">MTA</orgName>
          <desc>
            <address>
              <addrLine>Széchenyi István sqr. 9, 1051 Budapest</addrLine>
              <country key="HU"/>
            </address>
            <ref type="url">http://mta.hu/english</ref>
          </desc>
        </org>
        <org type="regrouplaboratory" xml:id="struct-54664" status="VALID">
          <orgName>Armand-Frappier Santé Biotechnologie Research Centre</orgName>
          <orgName type="acronym">INRS-AFSB</orgName>
          <date type="start">1938-01-01</date>
          <desc>
            <address>
              <addrLine>531 boul. des Prairies Laval (Québec) H7V 1B7</addrLine>
              <country key="CA"/>
            </address>
            <ref type="url">https://inrs.ca/en/inrs/research-centres/armand-frappier-sante-biotechnologie-research-centre/</ref>
          </desc>
          <listRelation>
            <relation active="#struct-92448" type="direct"/>
            <relation active="#struct-301247" type="direct"/>
          </listRelation>
        </org>
        <org type="institution" xml:id="struct-92448" status="VALID">
          <idno type="ROR">https://ror.org/04td37d32</idno>
          <orgName>Institut National de la Recherche Scientifique [Québec]</orgName>
          <orgName type="acronym">INRS</orgName>
          <desc>
            <address>
              <addrLine>490 rue de la Couronne, Québec, QC G1K 9A9</addrLine>
              <country key="CA"/>
            </address>
            <ref type="url">http://www.inrs.ca/</ref>
          </desc>
        </org>
        <org type="regroupinstitution" xml:id="struct-301247" status="VALID">
          <orgName>Pasteur Network (Réseau International des Instituts Pasteur)</orgName>
          <desc>
            <address>
              <addrLine>25, rue du Dr Roux 75724 Paris Cedex 15</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">https://pasteur-network.org/</ref>
          </desc>
        </org>
      </listOrg>
    </back>
  </text>
</TEI>