%0 Journal Article %T Development of molecular and cellular tools to decipher the type I IFN pathway of the common vampire bat %+ Laboratoire des Interactions Virus-Hôtes [Cayenne, Guyane Française] %+ Hépacivirus et immunité innée %A Sarkis, Sarkis %A Lise, Marie-Claude %A Darcissac, Edith %A Dabo, Stéphanie %A Falk, Marcel %A Chaulet, Laura %A Neuveut, Christine %A Meurs, Eliane %A Lavergne, Anne %A Lacoste, Vincent %Z This study was conducted within the CAROLIA and BATIMMUNE programs. CAROLIA was supported by European funds (ERDF/FEDER) and assistance from the Region Guyane and the Direction Regionale pour la Recherche et la Technologie. BATIMMUNE wasfunded by the Institut Pasteur through a Transversal Research Program (PTR499). This study also received a European Commission "REGPOT-CT-2011-285837-STRonGer" grant within the FP7 and an "Investissement d'Avenir " grant managed by the Agence Nationale de la Recherche (CEBA, Ref. ANR-10-LABEX-25-01). %< avec comité de lecture %@ 0145-305X %J Developmental and Comparative Immunology %I Elsevier %V 81 %P 1 - 7 %8 2017-11-06 %D 2017 %R 10.1016/j.dci.2017.10.023 %M 29122634 %K Type I interferon %K Cell line %K Desmodus rotundus %K Innate immunity %K Toll-like receptor %K RIG-I-Like receptor %Z Life Sciences [q-bio]/Immunology/Innate immunity %Z Life Sciences [q-bio]/Microbiology and Parasitology/VirologyJournal articles %X Though the common vampire bat, Desmodus rotundus, is known as the main rabies virus reservoir in Latin America, no tools are available to investigate its antiviral innate immune system. To characterize the IFN-I pathway, we established an immortalized cell line from a D. rotundus fetal lung named FLuDero. Then we molecularly characterized some of the Toll-like receptors (TLR3, 7, 8 and 9), the three RIG-I-like receptor members, as well as IFNa1 and IFNb. Challenging the FLuDero cell line with poly (I:C) resulted in an up-regulation of both IFNa1 and IFNb and the induction of expression of the different pattern recognition receptors characterized. These findings provide evidence of the intact dsRNA recognition machinery and the IFN-I signaling pathway in our cellular model. Herein, we generated a sum of insightful specific molecular and cellular tools that will serve as a useful model to study virusehost interactions of the common vampire bat. %G English %2 https://riip.hal.science/pasteur-01633796/document %2 https://riip.hal.science/pasteur-01633796/file/2018%20Sarkis%20et%20al%20DCI.pdf %L pasteur-01633796 %U https://riip.hal.science/pasteur-01633796 %~ PASTEUR %~ RIIP %~ CNRS %~ RIIP_GUYANE %~ OPENAIRE %~ ANR