%0 Journal Article %T Anti-angiogenic effect of phospholipases A 2 from Scorpio maurus venom glands on Human Umbilical Vein Endothelial Cells %+ École Nationale d'Ingénieurs de Sfax | National School of Engineers of Sfax (ENIS) %+ Laboratoire des Venins et Biomolécules Thérapeutiques - Laboratory of Venoms and Therapeutic Biomolecules (LR11IPT08) %+ Centre de Recherches en Oncologie biologique et Oncopharmacologie (CRO2) %+ Institut de neurophysiopathologie (INP) %A Krayem, Najeh %A Abdelkefi-Koubaa, Zaineb %A Marrakchi, Naziha %A Luis, José %A Gargouri, Youssef %< avec comité de lecture %@ 0041-0101 %J Toxicon %I Elsevier %V 145 %P 6-14 %8 2018-04 %D 2018 %R 10.1016/j.toxicon.2018.02.042 %M 29486161 %K Adhesion %K Anti-angiogenic effect %K Heterodimeric phospholipase A(2) %K Invasion %K Migration %K Scorpion venom glands %Z Life Sciences [q-bio]Journal articles %X In a previous study, we purified Sm-PLGV an heterodimeric phospholipase A2, from the venom glands of the Tunisian scorpion Scorpio maurus. This enzyme contains a Long chain, a penta-peptide insertion, which is cut out during the maturation process, followed by a short chain. A disulfide bridge links the two chains. Three recombinant forms of this enzyme were produced in Escherichia coli: rPLA2(+5) with a penta-peptide insert, rPLA2(-5) without the penta-peptide, and the Long chain alone without the short one. In the present study, we showed that Sm-PLGV, rPLA2(+5) and rPLA2(-5) displayed more potent anti-angiogenic activity in vitro than the recombinant Long chain and the short one obtained by chemical synthesis. These phospholipases A2 inhibited in a dose-dependent manner adhesion, migration and invasion of Human Umbilical Vein Endothelial Cells. Using Matrigel™, we demonstrated that Sm-PLGV, rPLA2(+5) and rPLA2(-5) significantly inhibited tubulogensesis. We also showed a clear dissociation between the anti-angiogenic effect of Sm-PLGV and its catalytic activity. %G English %L pasteur-01954950 %U https://riip.hal.science/pasteur-01954950 %~ RIIP %~ CNRS %~ UNIV-AMU %~ RIIP_TUNIS %~ INP %~ INP-CSNP %~ PHARMA-AMU