%0 Journal Article %T Snake venomics: Comparative analysis of the venom proteomes of the Tunisian snakes Cerastes cerastes, Cerastes vipera and Macrovipera lebetina %+ Laboratoire des Venins et Toxines, Institut Pasteur de Tunis %+ Consejo Superior de Investigaciones Cientificas = Spanish National Research Council (CSIC) %A Bazaa, Amine %A Marrakchi, Naziha %A El Ayeb, Mohamed %A Sanz, Libia %A Calvete, Juan %Z This study has been partly financed by grant BFU2004–01432/BMC from the Ministerio de Educación y Ciencia, Madrid, Spain, and by the Spanish-Tunisian Cooperation Programme 29p/02 from the Agencia Española de Cooperación Internacional (AECI). %< avec comité de lecture %@ 1615-9853 %J Proteomics %I Wiley-VCH Verlag %V 5 %N 16 %P 4223-4235 %8 2005-11 %D 2005 %R 10.1002/pmic.200402024 %M 16206329 %K Cerastes vipera %K Macrovipera lebetina %K N‐terminal sequencing %K Snake venomics %K Cerastes cerastes %Z Life Sciences [q-bio]/Biochemistry, Molecular Biology %Z Life Sciences [q-bio]/ToxicologyJournal articles %X The protein composition of the crude venoms of the three most important vipers of Tunisia was analyzed by RP-HPLC, N-terminal sequence analysis, MALDI-TOF mass determination, and in-gel tryptic digestion followed by PMF and CID-MS/MS of selected peptide ions in a quadrupole-linear IT instrument. Our results show that the venom proteomes of Cerastes cerastes, Cerastes vipera, and Macrovipera lebetina are composed of proteins belonging to a few protein families. However, each venom showed distinct degree of protein composition complexity. The three venoms shared a number of protein classes though the relative occurrence of these toxins was different in each snake species. On the other hand, the venoms of the Cerastes species and Macrovipera lebetina each contained unique components. The comparative proteomic analysis of Tunisian snake venoms provides a comprehensible catalogue of secreted proteins, which may contribute to a deeper understanding of the biological effects of the venoms, and may also serve as a starting point for studying structure-function %G English %Z The authors wish to thank Dr. Ben Lasfar Z. from Pasteur Institute of Tunis for providing the venoms. %L pasteur-02018971 %U https://riip.hal.science/pasteur-02018971 %~ RIIP %~ RIIP_TUNIS