%0 Journal Article %T Effects of metal ions on cyprinid fish immune response: In vitro effects of Zn2+ and Mn2+ on the mitogenic response of carp pronephros lymphocytes %+ Laboratoire d'Immunologie, Institut Pasteur de Tunis %+ Laboratoire d'Immunophysiologie Générale et Comparée %A Ghanmi, Z. %A Rouabhia, M. %A Othmane, O. %A Deschaux, P.A. %Z This study was supported by Environmental Ministry Grant 237 0 1 87 40021. %< avec comité de lecture %@ 0147-6513 %J Ecotoxicology and Environmental Safety %I Elsevier %V 17 %N 2 %P 183-189 %8 1989-04 %D 1989 %R 10.1016/0147-6513(89)90037-7 %M 2737112 %Z Life Sciences [q-bio]Journal articles %X Lymphocytes from the pronephros of carp (Cyprinus carpio L) have been subjected to transformation by mitogens, concanavalin A (Con A), phytohemagglutinin (PHA), and lipopolysaccharides (LPS), with Zn or Mn at varying concentrations. Addition of Zn2+ (10−7 to 10−3 M) to mitogen-stimulated T and B cells enhanced [3H]thymidine incorporation. Addition of 10−5 M Zn2+ inhibited the response to Con A, PHA, and LPS. At this concentration, Zn was toxic. Addition of Mn2+ (10−7 to 10−3 M) to mitogen-stimulated lymphocytes enhanced [3H]thymidine incorporation. This effect was observed with Con A- and PHA-stimulated lymphocytes, but not with LPS-stimulated lymphocytes. In contrast, addition of 10−1 M Mn2+ to lymphocyte cultures exerted an inhibitor effect on the response to Con A or to PHA, while the response to LPS was unaffected. Addition of 10−1 M Mn2+ to Con A- or PHA-stimulated cultures at different times after initiation of stimulation indicated that Mn2+ was inhibitory only when it was added before the first 16 hr of cultures. The inhibition induced by 10−1 M Mn2+ could be reversed by adding 2 mM CaCl2 to the culture. %G English %Z We thank 0. Nardou and J. C. Fage for technical assistance %L pasteur-02043718 %U https://riip.hal.science/pasteur-02043718 %~ UNILIM %~ RIIP %~ RIIP_TUNIS