%0 Unpublished work %T Rapid metagenomic characterization of a case of imported COVID-19 in Cambodia %+ National Institute of Allergy and Infectious Diseases [Phnom Penh, Cambodia] (NIAID) %+ Ministry of Health [Phnom Penh] %+ Chan Zuckerberg Initiative [Redwood City, CA] (CZI) %+ Chan Zuckerberg BioHub [San Francisco, CA] %+ Institut Pasteur du Cambodge %+ Unité de Virologie / Virology Unit [Phnom Penh] %A Manning, Jessica, E. %A Bohl, Jennifer, A. %A Lay, Sreyngim %A Chea, Sophana %A Sovann, Ly %A Sengdoeurn, Yi %A Heng, Seng %A Vuthy, Chan %A Kalantar, Katrina %A Ahyong, Vida %A Tan, Michelle %A Sheu, Jonathan %A Tato, Cristina, M. %A Derisi, Joseph, L. %A Baril, Laurence %A Duong, Veasna %A Dussart, Philippe %A Karlsson, Erik, A. %Z The work of Dr. Manning is supported by the Division of Intramural Research at the National Institute of Allergy and Infectious Diseases at the National Institutes of Health and the Bill and Melinda Gates Foundation. The work of Dr. Karlsson is supported, in part, by , by the U.S. Department of Health and Human Services, Office of the Assistant Secretary for Preparedness and Response (Grant No. 1 IDSEP190051-01-00) and through internal funding at Institut Pasteur in Cambodia. %8 2021-05-09 %D 2021 %R 10.1101/2020.03.02.968818 %M 32511296 %K metagenomics %K sequencing %K coronavirus %K COVID-19 %K SARS-CoV-2 %K unbiased metagenomics %Z Life Sciences [q-bio]/Microbiology and Parasitology/VirologyPreprints, Working Papers, ... %X Rapid production and publication of pathogen genome sequences during emerging disease outbreaks provide crucial public health information. In resource-limited settings, especially near an outbreak epicenter, conventional deep sequencing or bioinformatics are often challenging. Here we successfully used metagenomic next generation sequencing on an iSeq100 Illumina platform paired with an open-source bioinformatics pipeline to quickly characterize Cambodia's first case of COVID-2019. %G English %2 https://riip.hal.science/pasteur-03221716/document %2 https://riip.hal.science/pasteur-03221716/file/Manning%20et%20al.%20-%202020%20-%20Rapid%20metagenomic%20characterization%20of%20a%20case%20of%20im.pdf %L pasteur-03221716 %U https://riip.hal.science/pasteur-03221716 %~ RIIP %~ RIIP_CAMBODGE