Stability-related studies on 17D yellow fever vaccine. - RIIP - Réseau International des Instituts Pasteur Access content directly
Journal Articles Microbes and Infection Year : 2000

Stability-related studies on 17D yellow fever vaccine.


Yellow fever (YF) vaccine using the 17D strain of YF attenuated virus has been produced at the Institut Pasteur in Dakar since 1962. Until now, the stabilised YF had an expiry date of utilization of two years from the end of the lot control process under storage at +4 degrees C. We conducted a stability study to assess the three full year validity of this preparation, when correctly stored at +4 degrees C to optimise the conditions of production, storage and availability of such a vaccine. The activity of 19 consecutive batches of vaccines kept for three years at +4 degrees C was compared to that of the same batches that were kept three years at -20 degrees C. Using the in vitro microculture method, we found that three-year storage at +4 degrees C induced a higher loss of activity than storage at -20 degrees C or than the accelerated degradation test of vaccines kept for 14 days at 37 degrees C. Whatever the conditions of storage, in all cases decreases in activity were below the WHO's requirements, i.e., < 1 log PFU/dose, and residual activity of the selected batches was over 1000 mouse LD50 per dose. We demonstrated that the 17D YF vaccine produced in Dakar has a shelf-life of three years and that its required potency was maintained at +4 degrees C, after reconstitution with saline diluent, following three-year storage at +4 degrees C.

Dates and versions

pasteur-00566865 , version 1 (17-02-2011)



R. Perraut, G. Girault, J. P. Moreau. Stability-related studies on 17D yellow fever vaccine.. Microbes and Infection, 2000, 2 (1), pp.33-8. ⟨10.1016/S1286-4579(00)00288-4⟩. ⟨pasteur-00566865⟩


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