Influenza A virus replication induces cell cycle arrest in G0/G1 phase. - RIIP - Réseau International des Instituts Pasteur Access content directly
Journal Articles Journal of Virology Year : 2010

Influenza A virus replication induces cell cycle arrest in G0/G1 phase.

Abstract

Many viruses interact with the host cell division cycle to favor their own growth. In this study, we examined the ability of influenza A virus to manipulate cell cycle progression. Our results show that influenza A virus A/WSN/33 (H1N1) replication results in G(0)/G(1)-phase accumulation of infected cells and that this accumulation is caused by the prevention of cell cycle entry from G(0)/G(1) phase into S phase. Consistent with the G(0)/G(1)-phase accumulation, the amount of hyperphosphorylated retinoblastoma protein, a necessary active form for cell cycle progression through late G(1) into S phase, decreased after infection with A/WSN/33 (H1N1) virus. In addition, other key molecules in the regulation of the cell cycle, such as p21, cyclin E, and cyclin D1, were also changed and showed a pattern of G(0)/G(1)-phase cell cycle arrest. It is interesting that increased viral protein expression and progeny virus production in cells synchronized in the G(0)/G(1) phase were observed compared to those in either unsynchronized cells or cells synchronized in the G(2)/M phase. G(0)/G(1)-phase cell cycle arrest is likely a common strategy, since the effect was also observed in other strains, such as H3N2, H9N2, PR8 H1N1, and pandemic swine H1N1 viruses. These findings, in all, suggest that influenza A virus may provide favorable conditions for viral protein accumulation and virus production by inducing a G(0)/G(1)-phase cell cycle arrest in infected cells.
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Dates and versions

pasteur-00624904 , version 1 (20-09-2011)

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Yuan He, Ke Xu, Bjoern Keiner, Jianfang Zhou, Volker Czudai, et al.. Influenza A virus replication induces cell cycle arrest in G0/G1 phase.. Journal of Virology, 2010, 84 (24), pp.12832-40. ⟨10.1128/JVI.01216-10⟩. ⟨pasteur-00624904⟩

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