Adaptation of the highly productive T7 expression system to Streptomyces lividans. - RIIP - Réseau International des Instituts Pasteur Access content directly
Journal Articles Applied and Environmental Microbiology Year : 2010

Adaptation of the highly productive T7 expression system to Streptomyces lividans.

Abstract

Streptomyces lividans is a Gram-positive bacterium known for its remarkable secretion efficiency and low extracellular protease activity. In the present work, we adapted the highly productive T7 expression system to S. lividans. A codon-optimized T7 RNA polymerase gene was chromosomally integrated, and a bifunctional T7 expression vector was constructed.

Dates and versions

pasteur-00819554 , version 1 (01-05-2013)

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François-Xavier Lussier, François Denis, François Shareck. Adaptation of the highly productive T7 expression system to Streptomyces lividans.. Applied and Environmental Microbiology, 2010, 76 (3), pp.967-70. ⟨10.1128/AEM.02186-09⟩. ⟨pasteur-00819554⟩

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