Modulation of the reactivity of the thiol of human serum albumin and its sulfenic derivative by fatty acids. - RIIP - Réseau International des Instituts Pasteur Access content directly
Journal Articles Archives of Biochemistry and Biophysics Year : 2012

Modulation of the reactivity of the thiol of human serum albumin and its sulfenic derivative by fatty acids.

Abstract

The single cysteine residue of human serum albumin (HSA-SH) is the most abundant plasma thiol. HSA transports fatty acids (FA), a cargo that increases under conditions of diabetes, exercise or adrenergic stimulation. The stearic acid-HSA (5/1) complex reacted sixfold faster than FA-free HSA at pH 7.4 with the disulfide 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB) and twofold faster with hydrogen peroxide and peroxynitrite. The apparent pK(a) of HSA-SH decreased from 7.9±0.1 to 7.4±0.1. Exposure to H(2)O(2) (2mM, 5min, 37°C) yielded 0.29±0.04mol of sulfenic acid (HSA-SOH) per mole of FA-bound HSA. The reactivity of HSA-SOH with low molecular weight thiols increased ∼threefold in the presence of FA. The enhanced reactivity of the albumin thiol at neutral pH upon FA binding can be rationalized by considering that the corresponding conformational changes that increase thiol exposure both increase the availability of the thiolate due to a lower apparent pK(a) and also loosen steric constraints for reactions. Since situations that increase circulating FA are associated with oxidative stress, this increased reactivity of HSA-SH could assist in oxidant removal.
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Dates and versions

pasteur-00839259 , version 1 (27-06-2013)

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María José Torres, Lucía Turell, Horacio Botti, Laura Antmann, Sebastián Carballal, et al.. Modulation of the reactivity of the thiol of human serum albumin and its sulfenic derivative by fatty acids.. Archives of Biochemistry and Biophysics, 2012, 521 (1-2), pp.102-10. ⟨10.1016/j.abb.2012.03.011⟩. ⟨pasteur-00839259⟩
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