Conserved nucleation sites reinforce the significance of Phi value analysis in protein-folding studies. - RIIP - Réseau International des Instituts Pasteur Access content directly
Journal Articles IUBMB Life Year : 2014

Conserved nucleation sites reinforce the significance of Phi value analysis in protein-folding studies.

Abstract

The only experimental strategy to address the structure of folding transition states, the so-called Φ value analysis, relies on the synergy between site directed mutagenesis and the measurement of reaction kinetics. Despite its importance, the Φ value analysis has been often criticized and its power to pinpoint structural information has been questioned. In this hypothesis, we demonstrate that comparing the Φ values between proteins not only allows highlighting the robustness of folding pathways but also provides per se a strong validation of the method.
Embargoed file
Embargoed file
Visibility date is undetermined
Loading...

Dates and versions

pasteur-01181367 , version 1 (30-07-2015)

Identifiers

Cite

Stefano Gianni, Per Jemth. Conserved nucleation sites reinforce the significance of Phi value analysis in protein-folding studies.. IUBMB Life, 2014, 66 (7), pp.449-52. ⟨10.1002/iub.1287⟩. ⟨pasteur-01181367⟩

Collections

RIIP
43 View
2 Download

Altmetric

Share

Gmail Mastodon Facebook X LinkedIn More