Model-free methods to study membrane environmental probes: a comparison of the spectral phasor and generalized polarization approaches. - RIIP - Réseau International des Instituts Pasteur Access content directly
Journal Articles Methods and Applications in Fluorescence Year : 2015

Model-free methods to study membrane environmental probes: a comparison of the spectral phasor and generalized polarization approaches.

Abstract

In this note, we present a discussion of the advantages and scope of model-free analysis methods applied to the popular solvatochromic probe LAURDAN, which is widely used as an environmental probe to study dynamics and structure in membranes. In particular, we compare and contrast the generalized polarization approach with the spectral phasor approach. To illustrate our points we utilize several model membrane systems containing pure lipid phases and, in some cases, cholesterol or surfactants. We demonstrate that the spectral phasor method offers definitive advantages in the case of complex systems.

Dates and versions

pasteur-01498362 , version 1 (29-03-2017)

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Leonel Malacrida, Enrico Gratton, David M Jameson. Model-free methods to study membrane environmental probes: a comparison of the spectral phasor and generalized polarization approaches.. Methods and Applications in Fluorescence, 2015, 3 (4), ⟨10.1088/2050-6120/3/4/047001⟩. ⟨pasteur-01498362⟩
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