Structural insights into the neutralization mechanism of a higher primate antibody against dengue virus - RIIP - Réseau International des Instituts Pasteur Access content directly
Journal Articles EMBO Journal Year : 2011

Structural insights into the neutralization mechanism of a higher primate antibody against dengue virus

Abstract

The four serotypes of dengue virus (DENV-1 to-4) cause the most important emerging viral disease. Protein E, the principal viral envelope glycoprotein, mediates fusion of the viral and endosomal membranes during virus entry and is the target of neutralizing antibodies. However, the epitopes of strongly neutralizing human antibodies have not been described despite their importance to vaccine development. The chimpanzee Mab 5H2 potently neutralizes DENV-4 by binding to domain I of E. The crystal structure of Fab 5H2 bound to E from DENV-4 shows that antibody binding prevents formation of the fusogenic hairpin conformation of E, which together with in-vitro assays, demonstrates that 5H2 neutralizes by blocking membrane fusion in the endosome. Furthermore, we show that human sera from patients recovering from DENV-4 infection contain antibodies that bind to the 5H2 epitope region on domain I. This study, thus, provides new information and tools for effective vaccine design to prevent dengue disease.

Keywords

Domains

Virology

Dates and versions

pasteur-03214527 , version 1 (01-05-2021)

Identifiers

Cite

Joseph Jb Cockburn, M Erika Navarro Sanchez, Ana P Goncalvez, Elena Zaitseva, Enrico A Stura, et al.. Structural insights into the neutralization mechanism of a higher primate antibody against dengue virus. EMBO Journal, 2011, 31 (3), pp.767 - 779. ⟨10.1038/emboj.2011.439⟩. ⟨pasteur-03214527⟩
11 View
1 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More